Miscellaneous Molecular Biology Reagents
New England Biolabs, Inc. SNAP-Capture Magnetic Beads – 2 ml
The SNAP-Capture Magnetic Beads are used to selectively immobilize and magnetically separate a SNAP-tag fusion protein from solution using magnetic agarose beads. They are prepared by the coupling of SNAP-tag substrate benzylguanine with highly stable 20-100 um superparamagnetic particles.
New England Biolabs, Inc. SspI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Endonuclease IV – 5000 units
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
Bioworld Agarose Gel Loading Dye (6X) with Ficol, 50 mL
Agarose Gel Loading Dye, 6X, with Ficoll Contains: Bromophenol Blue 0,25%, Xylene Cynol FF 0.25% and Ficol 15% in 3D sterile water. Gel loading dye for molecular biology. Technical Information: 6X concentration Bromophenol Blue (0.25%) Xylene cyanol FF (0.25%) Ficoll in water (15%) Storage: 2-8C
Enzo Life Sciences FLUOR DE LYS developer I concentrate (300 µl)
An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. FLUOR DE LYS Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. An appropriate FLUOR DE LYS family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins). Shipping: Shipped on Blue Ice. Long Term Storage: -80°C
Ihc World LLC IHC-TEK EPITOPE SOLUTION 1L
This Newly Formulated Solution Is To Be Used Universally For Retrieving Antigens And Epitopes Of All Types, Including Membrane Proteins, Cytoplasmic Proteins As Well As Nuclear Proteins, On Formalin Fixed Paraffin Embedded Tissue Sections During Immunohistochemistry Procedure. This Solution Is Superior To Commonly Used Citrate Based Solutions And Less Tissue Destruction Than Other High Ph Solutions.
Hampton Research HR3-133/I3C Phasing Kit - 12 aliquots of I3C (280 mg each) and 12 aliquots of 2.0 M Lithium hydroxide (650 ul each)
HR3-133/I3C Phasing Kit - 12 aliquots of I3C (280 mg each) and 12 aliquots of 2.0 M Lithium hydroxide (650 ul each). Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences MES Monohydrate | 145224-94-8 | MFCD00149409 | 10g
MES Monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 10g
Teco Diagnostics BUN REAGENT SET
For the direct colorimetric determination of Urea Nitrogen (BUN) in human serum or plasma. 2x100mL Vials of BUN Enzyme Reagent (Color), 2x100mL Vials of BUN Color Developer, 1x5mL Vial of BUN Standard,
Quidel Corporation MicroVue BAP EIA Assay Kit
The MicroVue BAP EIA provides a quantitative measure of bone-specific alkaline phosphatase (BAP) activity in serum.
Molecular Dimensions CRYSTALLOPHORE NO1 5X0.6MG
Crystallophore No1 (patent pending) is a luminescent Terbium complex that is an effective nucleant and provides significant isomorphous and anomalous phasing power. This is the 5 vial multipack version of this product.
Cosmo Bio Usa Inc Lymphoprep 250ml
Lymphoprep™ is a ready-made, sterile, and endotoxin tested solution that has been used to isolate mononuclear cells for more than 35 years. Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an iso-osmotic medium with a density of 1.077 g/ml. Mononuclear cells are therefore retained at the sample/medium interface while erythrocytes and PMNs sediment through the medium.This method is rapid, simple, and reliable and gives excellent results with blood samples from normal individuals and patients.
MOLTOX Molecular Toxicology Inc NADPH REGENSYS A, 40 mL size refers to final volume of S9 mix per pkg, G-6-P MgCl2-KCL in 0.1M Phosphate Buffer, pH 7.4, Store in Refrigerator
Convenient preformulated filter sterilized and prepackaged S9 mix components for preparation of 4 - 10% S9 mix. Regensys™ """"A"""" is formulated as described by Maron & Ames, Mut. Res. 113: 173, 1983. Size refers to final volume of S9 mix. Regensys™ """"B"""" consists of accurately weighed aliquots of crystalline NADP to add to corresponding Regensys™ """"A"""" for completion of the Regensys™ system. MOLTOX S9 preparations and cofactor reagents for use in metabolic activation studies are manufactured to rigorous standards of quality and performance and are used by leading industrial, government and academic laboratories worldwide.
Sigma Aldrich Fine Chemicals Biosciences beta-Estradiol analytical standard | 50-28-2 | MFCD00003693 | 1VL
beta-Estradiol analytical standard | Mol Wt: 272.38 | 50-28-2 | MFCD00003693 | 1VL